| Enzyme Applications | کاربردهای آنزیم | Selective release of high mannose and biantennary complex type N-linked oligosaccharides from glycopeptides/ glycoproteins. |
|---|---|---|
| Enzyme Formulation | فرمولاسیون آنزیم | 10 mM sodium acetate pH 4.5, 25 mM NaCl, 0.02% sodium azide |
| Enzyme Source | منبع آنزیم | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. |
| Enzyme Specificity | ویژگی اختصاصی آنزیم | The enzyme cleaves within the di-N-acetyl chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-linked glycans of oligosaccharide, glycopeptide, or glycoprotein substrates. A single GlcNAc residue remains attached to the molecule after hydrolysis, the rest of the glycan is released. This specificity is distinct from PNGase F (GKE-5006), which cleaves between asparagine and the innermost GlcNAc of N-linked glycans. EndoF2 cleaves between GlcNAc-GlcNac in biantennary glycans at a rate approximately 20 times higher than high mannose glycans. The enzyme is not active towards hybrid type or tri- and tetraantennary oligosaccharides. |
| Enzyme Unit Definition | تعریف واحد آنزیم | One milliunit is defined as the amount of enzyme that will hydrolyze 1 nM of the dansylated porcine fibrinogen glycopeptide substrate DnsVGEN(CHO)R per minute at pH 4.5 and 37°C. |
| Unit | واحد | 6 mU |
| Volume | حجم | 60 µL |
| pH Optimum | pH بهینه | 4.5 |
Agilent AdvanceBio Endo F2, Recombinant (GKE-5008)
Part no: GKE-5008
AdvanceBio Endo F2, recombinant. Enzyme cleaves within the chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-glycans. Includes 5X reaction buffer, which when diluted gives 200 mM sodium acetate pH 4.5.
برای مطالعهی جزئیات بیشتر دربارهی قطعه با پارت نامبر GKE-5008 به بخش مشخصات فنی در همین صفحه مراجعه نمایید.
مشخصات محصول
| رده | کروماتوگرافی (Chromatography) |
|---|---|
| دستهبندی دستگاهی | کروماتوگرافی مایع (Liquid Chromatography) |
| نوع تجهیز | قطعات و لوازم جانبی |
| قطعات HPLC | سایر |
| وضعیت | نو |
| برند | Agilent |
| موجودی | موجود |
| کشور سازنده | امریکا |
| گارانتی | ندارد |
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