| Concentration | غلظت | ≥2.5 U/mL |
|---|---|---|
| Enzyme Applications | کاربردهای آنزیم |
|
| Enzyme Formulation | فرمولاسیون آنزیم | 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5) |
| Enzyme Source | منبع آنزیم | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. The enzyme is also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. |
| Enzyme Specific Activity | فعالیت ویژه آنزیم | ≥10 units/mg |
| Enzyme Specificity | ویژگی اختصاصی آنزیم | Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free. |
| Enzyme Unit Definition | تعریف واحد آنزیم | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5. |
| Molecular Weight | وزن مولکولی | ~35,000 daltons |
| Unit | واحد | 1000 mU |
| Volume | حجم | 80 µL |
| pH Optimum | pH بهینه | 8.6 |
| pH Range | بازهی pH | 7.5-9.5 |
Agilent AdvanceBio N-Glycanase, 5x >=2.5 U/mL Vials (GKE-5006B-1)
Part no: GKE-5006B-1
AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL. A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Supplied as 5 x 200 mU vials of GKE-5006B. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).
برای مطالعهی جزئیات بیشتر دربارهی قطعه با پارت نامبر GKE-5006B-1 به بخش مشخصات فنی در همین صفحه مراجعه نمایید.
مشخصات محصول
| رده | کروماتوگرافی (Chromatography) |
|---|---|
| دستهبندی دستگاهی | کروماتوگرافی مایع (Liquid Chromatography) |
| نوع تجهیز | قطعات و لوازم جانبی |
| قطعات HPLC | سایر |
| وضعیت | نو |
| برند | Agilent |
| موجودی | موجود |
| کشور سازنده | امریکا |
| گارانتی | ندارد |
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