| Concentration | غلظت | 2.5 U/mL |
|---|---|---|
| Enzyme Formulation | فرمولاسیون آنزیم | 20 mM Tris-HCl, 50 mM NaCl (pH 7.5) |
| Enzyme Source | منبع آنزیم | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. |
| Enzyme Specific Activity | فعالیت ویژه آنزیم | ≥10 units/mg |
| Enzyme Specificity | ویژگی اختصاصی آنزیم | Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions. |
| Enzyme Unit Definition | تعریف واحد آنزیم | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5. |
| Molecular Weight | وزن مولکولی | ~35,000 Daltons |
| Unit | واحد | 1 U |
| Volume | حجم | 400 µL |
| pH Optimum | pH بهینه | 8.6 |
| pH Range | بازهی pH | 7.5-9.5 |
Agilent AdvanceBio N-Glycanase, EDTA-Free, >=2.5 U/mL (GKE-5016D)
Part no: GKE-5016D
AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL . A recombinant form of PNGase F (1U, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Does not include reaction buffers that ship with ‘A’ (100 mU) and ‘B’ (200 mU) pack sizes. Please contact us if you require buffers.
برای مطالعهی جزئیات بیشتر دربارهی قطعه با پارت نامبر GKE-5016D به بخش مشخصات فنی در همین صفحه مراجعه نمایید.
مشخصات محصول
| رده | کروماتوگرافی (Chromatography) |
|---|---|
| دستهبندی دستگاهی | کروماتوگرافی مایع (Liquid Chromatography) |
| نوع تجهیز | قطعات و لوازم جانبی |
| قطعات HPLC | سایر |
| وضعیت | نو |
| برند | Agilent |
| موجودی | موجود |
| کشور سازنده | امریکا |
| گارانتی | ندارد |
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