| Application | کاربرد | Detection of O-glycosylation in proteins |
|---|---|---|
| Concentration | غلظت | ≥1.25 U/mL |
| Enzyme Applications | کاربردهای آنزیم |
|
| Enzyme Formulation | فرمولاسیون آنزیم | 20 mM Tris HCl pH 7.5 containing 25 mM NaCl. |
| Enzyme Source | منبع آنزیم | Recombinant gene from Streptococcus pneumoniae, expressed in E.coli. |
| Enzyme Specific Activity | فعالیت ویژه آنزیم | ≥12 units/mg |
| Enzyme Specificity | ویژگی اختصاصی آنزیم | The enzyme is highly specific, liberating Galß1-3GalNAc from serine or threonine residues alone or as part of glycopeptides or glycoproteins. There is no apparent preference for serine over threonine. Substitution of the disaccharide core with sialic acid, fucose, N-acetylglucosamine or N-acetylgalactosamine residues, prevents cleavage of the Galß1-3GalNAc-Ser/Thr linkage. Absence of the galactose residue or the acetamido group on the innermost N-acetylgalactosamine residue also prevents cleavage of the Galß1-3GalNAc-Ser/Thr linkage. O-Glycanase is included in the Agilent Enzymatic Deglycosylation kit for N-linked and simple O-linked glycans (GK80110), which may be used in conjunction with our the Agilent Extender kit for complex O-linked glycans (GK80115). |
| Enzyme Unit Definition | تعریف واحد آنزیم | One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of Galß1-3GalNAca1-p-nitrophenol per minute at 37°C, pH 5.5. |
| Molecular Weight | وزن مولکولی | ~180 kD |
| Unit | واحد | 50 mU |
| Volume | حجم | 40 µL |
| pH Optimum | pH بهینه | 5.5 |
| pH Range | بازهی pH | 5-7 |
Agilent AdvanceBio O-Glycanase (GK80090)
Part no: GK80090
AdvanceBio O-Glycanase. Enzyme releases unsubstituted Ser/Thr-linked GalGalNAc from proteins and can be used with additional enzymes to remove more complex O-linked structures. Also known as Endo-O-Glycosidase, Endo-α N-acetylgalactosaminidase, O-Glycosidase. Includes 5X reaction buffer A [250 mM sodium phosphate pH 5.0]
برای مطالعهی جزئیات بیشتر دربارهی قطعه با پارت نامبر GK80090 به بخش مشخصات فنی در همین صفحه مراجعه نمایید.
مشخصات محصول
| رده | کروماتوگرافی (Chromatography) |
|---|---|
| دستهبندی دستگاهی | کروماتوگرافی مایع (Liquid Chromatography) |
| نوع تجهیز | قطعات و لوازم جانبی |
| قطعات HPLC | سایر |
| وضعیت | نو |
| برند | Agilent |
| موجودی | موجود |
| کشور سازنده | امریکا |
| گارانتی | ندارد |
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