| Enzyme Formulation | فرمولاسیون آنزیم | Lyophilized powder containing citrate-phosphate buffer, pH 5.0. |
|---|---|---|
| Enzyme Source | منبع آنزیم | Almond. Enzyme also knows as Peptide N-Glycosidase A, Glycopeptidase from almonds, Almond glycoamidase, Peptide-N(4)-(N-acetyl-β glucosaminyl) asparagine amidase. |
| Enzyme Specific Activity | فعالیت ویژه آنزیم | > 5 units/mg |
| Enzyme Specificity | ویژگی اختصاصی آنزیم | PNGase A from almonds cleaves N-glycan chains linked to Asparagine from glycopeptides. The enzyme hydrolyzes an N(4)-(acetyl-ß D-glucosaminyl) asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-ß D-glucosaminylamine and a peptide containing an aspartate residue. The enzyme cleaves N-glycans with or without a(1,3)-linked core fucose residues present in insect and plant glycoproteins. This differs from PNGase F, which cannot remove N-glycans with a(1,3)-linked core fucose. PNGase A requires glycopeptides as substrates. Glycopeptides can be obtained by digesting the glycoprotein with trypsin [1,2] or pepsin. Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino acid residues in the substrate. |
| Enzyme Unit Definition | تعریف واحد آنزیم | One unit is defined as the amount of enzyme required to hydrolyze 1 µmol ovalbumin glycopeptide per min at pH 5.0 and 37°C. |
| Molecular Weight | وزن مولکولی | 54.2 and 21.2 kDa |
| Unit | واحد | 10 mU |
| pH Optimum | pH بهینه | 5 |
| pH Range | بازهی pH | 4-6 |
Agilent AdvanceBio PNGase A (Almond), 10 mU (GKE-5011B)
Part no: GKE-5011B
AdvanceBio PNGase A (almond), 10 mU. Enzyme cleaves N-linked glycans from glycopeptides, including glycans with α(1,3)-linked core fucose as seen in insects and plants. Includes reaction buffer: 50 mM citrate-phosphate buffer, pH 5.0, 0.02% sodium azide.
برای مطالعهی جزئیات بیشتر دربارهی قطعه با پارت نامبر GKE-5011B به بخش مشخصات فنی در همین صفحه مراجعه نمایید.
مشخصات محصول
| رده | کروماتوگرافی (Chromatography) |
|---|---|
| دستهبندی دستگاهی | کروماتوگرافی مایع (Liquid Chromatography) |
| نوع تجهیز | قطعات و لوازم جانبی |
| قطعات HPLC | سایر |
| وضعیت | نو |
| برند | Agilent |
| موجودی | موجود |
| کشور سازنده | امریکا |
| گارانتی | ندارد |
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