| Concentration | غلظت | 5 U/mL |
|---|---|---|
| Enzyme Formulation | فرمولاسیون آنزیم | 20 mM Tris HCl pH 7.5, containing 25 mM NaCl. |
| Enzyme Source | منبع آنزیم | Recombinant gene from Arthrobacter ureafaciens, expressed in E. coli. Sialidases are also known as neuraminidases. |
| Enzyme Specific Activity | فعالیت ویژه آنزیم | 40 units/mg |
| Enzyme Specificity | ویژگی اختصاصی آنزیم | The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase. |
| Enzyme Unit Definition | تعریف واحد آنزیم | One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5. |
| Molecular Weight | وزن مولکولی | ~88 kD |
| Unit | واحد | 1 U |
| Volume | حجم | 200 µL |
| pH Optimum | pH بهینه | 6 |
Agilent AdvanceBio Sialidase A, 1 U (GK80040)
Part no: GK80040
AdvanceBio Sialidase A, 1 U. Enzyme releases α(2,3)-, α(2,6)-, α(2,8)-, and α(2,9)-linked N-acetylneuraminic acid from oligosaccharides and glycoproteins. Also capable of releasing N-glycolylneuraminic acid. Incudes 5x Reaction Buffer B [250 mM sodium phosphate pH 6.0] which when diluted gives 50 mM sodium phosphate pH 6.0.
برای مطالعهی جزئیات بیشتر دربارهی قطعه با پارت نامبر GK80040 به بخش مشخصات فنی در همین صفحه مراجعه نمایید.
مشخصات محصول
| رده | کروماتوگرافی (Chromatography) |
|---|---|
| دستهبندی دستگاهی | کروماتوگرافی مایع (Liquid Chromatography) |
| نوع تجهیز | قطعات و لوازم جانبی |
| قطعات HPLC | سایر |
| وضعیت | نو |
| برند | Agilent |
| موجودی | موجود |
| کشور سازنده | امریکا |
| گارانتی | ندارد |
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