| Concentration | غلظت | 5 U/mL |
|---|---|---|
| Enzyme Source | منبع آنزیم | Recombinant gene from Arthrobacter ureafaciens expressed in E. coli. Sialidases are also known as neuraminidases. |
| Enzyme Specificity | ویژگی اختصاصی آنزیم | The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase. |
| Enzyme Unit Definition | تعریف واحد آنزیم | One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5 |
| pH Optimum | pH بهینه | 6 |
Agilent AdvanceBio Sialidase A Sampler Kit (GK80043)
Part no: GK80043
AdvanceBio Sialidase A, Sampler Kit. Contains lower MW sialidase A isoforms for challenging sialidase digests. This sampler kit contains 1 vial each of 3 isoforms (88, 66 and 51 kDa) of the enzyme Sialidase A. Lower MW sialidases may help desialylate glycans if steric hindrance is an issue with regular sialidase.Each enzyme vial contains 200 mU in 40 µl. Also included is one vial 5x reaction buffer (1 mL). These products are available for purchase separately in 1-Unit pack sizes under the individual product codes GK80040 (88 kDa), GK80046 (66 kDa) and GK80045 (51 kDa).Sialidases are also known as neuraminidases.
برای مطالعهی جزئیات بیشتر دربارهی قطعه با پارت نامبر GK80043 به بخش مشخصات فنی در همین صفحه مراجعه نمایید.
مشخصات محصول
| رده | کروماتوگرافی (Chromatography) |
|---|---|
| دستهبندی دستگاهی | کروماتوگرافی مایع (Liquid Chromatography) |
| نوع تجهیز | قطعات و لوازم جانبی |
| قطعات HPLC | سایر |
| وضعیت | نو |
| برند | Agilent |
| موجودی | موجود |
| کشور سازنده | امریکا |
| گارانتی | ندارد |
Related products
فرول GC برند Agilent پارت نامبر 5062-3504
Part no: 5062-3504
SPLIT VENT TRAP دستگاه GC مدل RDT-1020
Part no: RDT-1020
فرول GC برند Agilent سازگار با PerkinElmer
Part no: 8003-0223
سرنگ اتوسمپلر GC برند Agilent مدل G4513-80228
Part no: G4513-80228
نظرات
There are no reviews yet.