| Concentration | غلظت | 150 U/mL |
|---|---|---|
| Enzyme Formulation | فرمولاسیون آنزیم | 20 mM Tris-HCl, 20 mM NaCl (pH 7.5) |
| Enzyme Source | منبع آنزیم | Jack bean. |
| Enzyme Specificity | ویژگی اختصاصی آنزیم | The enzyme has broad specificity, cleaving a(1-2,3,6)-linked mannose, although some kinetic preference has been observed (a1-2, 6 > 3). The enzyme will not cleave a single a(1-6) linked mannose residue from core ß-mannose, but will, however, remove a single a1-3 linked mannose from the core ß-mannose. By using enzyme concentrations of around 50 U/ml and extended incubation times (up to 18 hours) at 37°C, complete removal of all nonreducing terminal a linked mannose residues may be achieved. To expedite glycan sequencing studies, the sluggish activity of GKX-5010 jack bean a-mannosidase toward a(1-6)-linked mannose residues can be overcome by using the enzyme with the a- mannosidase from Xanthomonas mannihotis which rapidly cleaves a(1-6) linkages. The X. mannihotis a-mannosidase may inhibit the action of other mannosidases if a branched (a1-6) mannose is present in the substrate, so is typically added after incubation of the substrate with Jack bean a-mannosidase (GKX-5010). |
| Enzyme Unit Definition | تعریف واحد آنزیم | One unit is defined as the amount of enzyme that will hydrolyze 1 µmole of pNP-α-mannopyranoside per minute at pH 4.5 and 37°C. |
| Molecular Weight | وزن مولکولی | ~190 kD |
| Unit | واحد | 10 U |
| Volume | حجم | 70 µL |
| pH Optimum | pH بهینه | 4.5 |
Agilent Alpha-(1-2,3,6)-Mannosidase (Jack Bean) (GKX-5010)
Part no: GKX-5010
α(1-2,3,6)-Mannosidase (jack bean). Enzyme releases nonreducing terminal α(1-2,3,6)-linked mannose from oligosaccharides. Includes incubation buffer [(500 mM sodium acetate, 10 mM zinc chloride (pH 5.0)].
برای مطالعهی جزئیات بیشتر دربارهی قطعه با پارت نامبر GKX-5010 به بخش مشخصات فنی در همین صفحه مراجعه نمایید.
مشخصات محصول
| رده | کروماتوگرافی (Chromatography) |
|---|---|
| دستهبندی دستگاهی | کروماتوگرافی مایع (Liquid Chromatography) |
| نوع تجهیز | قطعات و لوازم جانبی |
| قطعات HPLC | سایر |
| وضعیت | نو |
| برند | Agilent |
| موجودی | موجود |
| کشور سازنده | امریکا |
| گارانتی | ندارد |
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