| Application | کاربرد | Structural analysis of O-glycan |
|---|---|---|
| Concentration | غلظت | ≥2 U/mL |
| Enzyme Applications | کاربردهای آنزیم |
|
| Enzyme Formulation | فرمولاسیون آنزیم | 52 mM Tris-HCl, 36 mM NaCl, 9 mM sodium acetate, 5 mM EDTA, 0.02% sodium azide (pH 7.5), glycerol-free formulation |
| Enzyme Source | منبع آنزیم | Native, purified from Elizabethkingia meningoseptica. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum |
| Enzyme Specificity | ویژگی اختصاصی آنزیم | PNGase F releases intact N-linked oligosaccharides from glycoproteins and glycopeptides. The enzyme will not cleave oligosaccharides from a single asparagine residue and cleavage does not occur if there is core α1-3 linked fucose as commonly encountered in plant glycoproteins Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. |
| Enzyme Unit Definition | تعریف واحد آنزیم | One unit of N-Glycanase is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at pH 7.5 and 37°C |
| Unit | واحد | 100 mU |
| Volume | حجم | 50 µL |
| pH Optimum | pH بهینه | 7.5 |
Agilent PNGase F, Chryseobacterium, Native (GKE-5003)
Part no: GKE-5003
PNGase F, Chryseobacterium, native. Native PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Also available as a recombinant product, AdvanceBio N-glycanse, see GKE-5006A. Includes 5x reaction buffer, denaturation solution, detergent solution.
برای مطالعهی جزئیات بیشتر دربارهی قطعه با پارت نامبر GKE-5003 به بخش مشخصات فنی در همین صفحه مراجعه نمایید.
مشخصات محصول
| رده | کروماتوگرافی (Chromatography) |
|---|---|
| دستهبندی دستگاهی | کروماتوگرافی مایع (Liquid Chromatography) |
| نوع تجهیز | قطعات و لوازم جانبی |
| قطعات HPLC | سایر |
| وضعیت | نو |
| برند | Agilent |
| موجودی | موجود |
| کشور سازنده | امریکا |
| گارانتی | ندارد |






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